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Table 4 Mean percentage of normal sperm morphology and intact plasma membrane after different treatments of semen

From: Enhancing canine semen quality through a second centrifugation after 48 hours of storage: a comparative study

Parameter (%)

Mean values ±

standard deviation

Group 1 (5–15 kg)

T0

 

T24

 

T48

AF

AD

AC

 

AD

AC

 

AD

AC

A2C

Normal morphology

82.23±

5.45 a

78.21±

4.73

84.47±

3.54 b

 

72.36±

5.60

80.26±

2.87 a

 

67.96±

5.53

75.37±

2.19 a

79.07±

2.14 a, c

Intact membrane

84.91±

2.56 a

81.35±

3.30

86.51±

1.76 b

 

77.08±

3.17

81.49±

1.78 a

 

73.18±

3.28

75.95±

3.12 a

79.01±

2.76 a, c

 

Group 2 (15–25 kg)

T0

 

T24

 

T48

AF

AD

AC

 

AD

AC

 

AD

AC

A2C

Normal morphology

82.65±

4.27 a

80.58±

4.14

86.21±

4.06 b

 

75.33±

3.67

81.07±

3.13 a

 

69.86±

3.94

75.07±

2.45 a

79.79±

2.48 a, c

Intact membrane

86.18±

3.07 a

83.30±

2.69

88.09±

2.38 b

 

78.74±

2.57

83.34±

2.18 a

 

73.98±

2.38

77.80±

2.58 a

79.89±

2.38 a, c

 

Group 3 (> 25 kg)

T0

 

T24

 

T48

AF

AD

AC

 

AD

AC

 

AD

AC

A2C

Normal morphology

80.69±

4.11 a

78.38±

4.40

84.03±

4.20 b

 

74.51±

4.23

79.63±

3.77 a

 

69.46±

4.18

74.14±

4.28 a

78.48±

3.75 a, c

Intact membrane

83.29±

2.65 a

80.67±

2.68

84.80±

2.58 b

 

76.64±

3.15

79.82±

2.58 a

 

71.99±

3.21

74.76±

2.91 a

77.02±

2.94 a

  1. T0 = moment of semen collection; T24 = after 24 h of refrigeration; T48 = after 48 h of refrigeration; AF=fresh semen; AD=after dilution; AC=after centrifugation; A2C = after second centrifugation
  2. a= statistically significant higher values than in AD (P < 0.005)
  3. b= statistically significant higher values in AC compared to AF and AD (P < 0.001)
  4. c= statistically significant higher values than Ac (P < 0.0001)