Table 1 Summary of immunohistochemical protocols used on tissue sections
From: Lumbar round cell sarcoma in a 10-week-old rottweiler puppy
Cell marker | Antibody type | Host | Source | Antigen retrievala | Dilution | Chromogen |
|---|---|---|---|---|---|---|
Vimentin | Monoclonal | Mouse | Dako | pH 8 | 1:64 | Ultraview ALP Redb |
Cytokeratin AE-1/AE-3 | Monoclonal | Mouse | Dako | pH 9 | 1:200 | Nova Redc |
Iba1 | Polyclonal | Rabbit | Wako | pH 8 | 1:2,000 | Ultraview ALP Red |
GFAPd | Polyclonal | Rabbit | Dako | PKe | 1:50 | Nova Red |
S100 | Monoclonal | Mouse | Dako | pH 8 | 1:2,000 | Ultraview ALP Red |
Ki-67 | Polyclonal | Rabbit | Intermedico | pH 8 | 1:100 | Ultraview ALP Red |
NSEe | Polyclonal | Rabbit | Abcam | pH 6 | 1:50 | Nova Red |
OLIG2f | Monoclonal | Rabbit | Sigma | pH 6 | 1:150 | Nova Red |
Pax5 | Polyclonal | Rabbit | Biocare | pH 9 | 1:50 | Nova Red |
CD3 | Polyclonal | Rabbit | Dako | pH 6 | 1:100 | Nova Red |
CD18 | Monoclonal | Mouse | UC Davis | pH 6 | 1:40 | Nova Red |